Mechanism of Inactivation of Bacteriophage J 1 by Dithiothreitol, Cysteine, Mercaptoethanol, or Thioglycollate
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چکیده
منابع مشابه
Mechanism of cysteine protease inactivation by peptidyl epoxides.
Peptidyl epoxides are time- and concentration-dependent selective cysteine protease inhibitors. The lack of recovery of enzymic activity and the retention of 1 molar equivalent of radioactive inhibitor associated with the enzyme on dialysis, shown in this study, indicate that they form a covalent irreversible equimolar complex with the enzyme. It is also shown that the peptidyl epoxide inhibito...
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A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0, and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubation temperature over 40'C result...
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When intact pea (Pisum sativum L.) plants are illuminated, the glycolytic enzyme phosphofructokinase is inactivated. In crude extracts the enzyme is inhibited by dithiothreitol. It would seem that this cytoplasmic enzyme, like glucose-6-P dehydrogenase, is light-inactivated when the enzymes of photosynthetic carbon metabolism are light-activated.
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15 صفحه اولMechanism of growth stimulation of L1210 cells by 2-mercaptoethanol in vitro. Role of the mixed disulfide of 2-mercaptoethanol and cysteine.
The mechanism of the growth-promoting action of 2-mercaptoethanol on mouse lymphoma L1210 cells in vitro has been studied. The normal line of L1210 is deficient in its capacity to take up cystine, and this limits the growth of the cells in normal culture media containing cystine. In the presence of 2-mercaptoethanol, the cells obtain a high capacity to utilize cystine in the medium. The reactio...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1973
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.37.2159